Guinea pig Visfatin (VF) ELISA Kit

Nampt ELISA Kit, NAmPRTase ELISA Kit, PBEF ELISA Kit, PBEF1 ELISA Kit, Pre-B-Cell Colony Enhancing Factor ELISA Kit, Nicotinamide Phosphoribosyltransferase ELISA Kit

Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig VF in the samples is then determined by comparing the OD of the samples to the standard curve