Mouse Cytochrome P450 27B1 (CYP27B1) ELISA Kit

VDD1, PDDR ELISA Kit, CYP1, P450c1 ELISA Kit, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase ELISA Kit, Cytochrome P450 Family 27 Subfamily B Polypeptide 1 ELISA Kit, Calcidiol 1-monooxygenase ELISA Kit, VD3 1A hydroxylase ELISA Kit

Standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP27B1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CYP27B1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CYP27B1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CYP27B1 in the samples is then determined by comparing the OD of the samples to the standard curve