Human Keratin 33A (KRT33A) ELISA Kit

KRTHA3A ELISA Kit, Ha-3I ELISA Kit, Krt1-3 ELISA Kit, K33A ELISA Kit, HHA3-I ELISA Kit, HKA3A ELISA Kit, Keratin, Hair, Acidic 3A ELISA Kit, Hard Keratin Type I 3I ELISA Kit, Keratin, type I cuticular Ha3-I ELISA Kit

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT33A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT33A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT33A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT33A in the samples is then determined by comparing the OD of the samples to the standard curve