Mouse Alpha-1-Microglobulin/Bikunin Precursor (a1M) ELISA Kit

AMBP ELISA Kit, UTI ELISA Kit, HCP ELISA Kit, EDC1 ELISA Kit, HI30 ELISA Kit, IATIL ELISA Kit, ITILC ELISA Kit, ITI ELISA Kit, ITIL ELISA Kit, Alpha 1 Microglobulin/Bikunin Precursor ELISA Kit, Growth-inhibiting protein 19 ELISA Kit, Uristatin ELISA Kit, Uronic-Acid-Rich Protein ELISA Kit, Trypstatin ELISA Kit

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse a1M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1M in the samples is then determined by comparing the OD of the samples to the standard curve.