Human Calcium Sensing Receptor (CASR) ELISA Kit

CAR ELISA Kit, FHH ELISA Kit, FIH ELISA Kit, GPRC2A ELISA Kit, HHC ELISA Kit, HHC1 ELISA Kit, NSHPT ELISA Kit, PCAR1 ELISA Kit, Parathyroid cell calcium-sensing receptor 1 ELISA Kit, Hypocalciuric Hypercalcemia 1 ELISA Kit, Severe Neonatal Hyperparathyroidism ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CASR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CASR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CASR in the samples is then determined by comparing the OD of the samples to the standard curve