Human Apoptosis Inhibitor 5 (API5) ELISA Kit

MIG8 ELISA Kit, FIF ELISA Kit, AAC11 ELISA Kit, API5L1 ELISA Kit, XAGL ELISA Kit, Cell migration-inducing gene 8 ELISA Kit, Antiapoptosis clone 11 ELISA Kit, Fibroblast Growth Factor 2-Interacting Factor 2 ELISA Kit, Migration-Inducing Protein MIG8 ELISA Kit

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human API5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human API5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human API5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human API5 in the samples is then determined by comparing the OD of the samples to the standard curve