Human Immediate Early Response 3 (IER3) ELISA Kit

PRG1 ELISA Kit, DIF-2 ELISA Kit, DIF2 ELISA Kit, GLY96 ELISA Kit, IEX-1 ELISA Kit, IEX-1L ELISA Kit, IEX1 ELISA Kit, Radiation-inducible immediate-early gene IEX-1 ELISA Kit, Differentiation-dependent gene 2 protein ELISA Kit, PACAP-responsive gene 1 protein ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IER3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IER3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IER3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IER3 in the samples is then determined by comparing the OD of the samples to the standard curve