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Human Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) .

Product Specifications

Product Name Alternative

CD148 ELISA Kit, PTPR-J ELISA Kit, DEP1 ELISA Kit, HPTPeta ELISA Kit, R-PTP-ETA ELISA Kit, SCC1 ELISA Kit, Protein-tyrosine phosphatase eta ELISA Kit, Density-enhanced phosphatase 1 ELISA Kit

UniProt

Q12913

Reactivity

Human

Field of Research

Pharmacology & Drug Discovery

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Sensitivity

0.116 ng/mL

Notes

For research use only.

Applications Notes

Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PTPRJ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PTPRJ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PTPRJ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PTPRJ in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.32-20 ng/mL

Available Sizes

Frequently Asked Questions

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