Human Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) ELISA Kit

PI3K ELISA Kit, PI3Kbeta ELISA Kit, PIK3C1 ELISA Kit, p110-BETA ELISA Kit, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIK3Cb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIK3Cb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIK3Cb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIK3Cb in the samples is then determined by comparing the OD of the samples to the standard curve