Human Serotonin Transporter (SERT) ELISA Kit

SLC6A4 ELISA Kit, SLC6-A4 ELISA Kit, HTT ELISA Kit, 5-HTT ELISA Kit, 5HTT ELISA Kit, OCD1 ELISA Kit, hSERT ELISA Kit, Solute Carrier Family 6, Member 4 ELISA Kit, Neurotransmitter Transporter, Serotonin ELISA Kit, Sodium-dependent serotonin transporter ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SERT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SERT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SERT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SERT in the samples is then determined by comparing the OD of the samples to the standard curve