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Human Calcyclin Binding Protein (CACYBP) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcyclin Binding Protein (CACYBP) .

Product Specifications

Product Name Alternative

SIP ELISA Kit, GIG5 ELISA Kit, PNAS-107 ELISA Kit, RP1-102G20.6 ELISA Kit, S100A6BP ELISA Kit, S100A6-binding protein ELISA Kit, Siah-interacting protein ELISA Kit

UniProt

Q9HB71

Reactivity

Human

Field of Research

Signal Transduction

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Sensitivity

33 pg/mL

Notes

For research use only.

Applications Notes

Standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CACYBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CACYBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CACYBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CACYBP in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

78.13-5000 pg/mL

Available Sizes

Frequently Asked Questions

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