Rat Interleukin 1 Receptor Antagonist (IL1RA) ELISA Kit

IL1RN ELISA Kit, IL1-RA ELISA Kit, ICIL1RA ELISA Kit, IL1ra3 ELISA Kit, IL1F3 ELISA Kit, IRAP ELISA Kit, IL-1RA ELISA Kit, Interleukin-1 Family Member 3 ELISA Kit, IL1 inhibitor ELISA Kit, Anakinra ELISA Kit

Standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1RA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1RA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1RA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1RA in the samples is then determined by comparing the OD of the samples to the standard curve