Human Chemokine C-X-C-Motif Receptor 3 (CXCR3) ELISA Kit

CD183 ELISA Kit, CKR-L2 ELISA Kit, CMKAR3 ELISA Kit, GPR9 ELISA Kit, IP10r ELISA Kit, IP10-R ELISA Kit, Mig-R ELISA Kit, MigR ELISA Kit, G Protein-Coupled Receptor 9 ELISA Kit, Interferon-inducible protein 10 receptor ELISA Kit, IP-10 receptor ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CXCR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CXCR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CXCR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CXCR3 in the samples is then determined by comparing the OD of the samples to the standard curve