Human Interleukin 1 Beta (IL1b) ELISA Kit

Kamila Pasternak-Mnich 1, Jolanta Kujawa 2, Justyna Agier 3, Elżbieta Kozłowska Impact of photobiomodulation therapy on pro-inflammation functionality of human peripheral blood mononuclear cells - a preliminary study Sci Rep, (2024), Rui Yuan 1, Qi-Chao Sun 2, Yue-Peng An 1, Qing Zhang 1, Qiong Zhang 1, Yue Li 3 Eriodictyol inhibits the proliferation and inflammatory response in keratinocytes in psoriasis through inactivating DYRK1A-mediated endoplasmic reticulum stress J Asian Nat Prod Res, (2025), P Cieplewicz-Guźla, MJ Kowalczyk The role of selected cytokines from the interleukin 1 family in the pathophysiology of morphea. researchsquare.com, (2025)

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve