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ORP9/OSBPL9 Antibody

Goat polyclonal antibody to OSBPL9

Product Specifications

Product Name Alternative

Anti OSBPL9 antibody, anti ORP9 antibody, anti FLJ12492 antibody, anti FLJ14629 antibody, anti FLJ14801 antibody, anti FLJ32055 antibody, anti FLJ34384 antibody, anti MGC15035 antibody, anti oxysterol binding protein-like 9 antibody, anti OSBP-related protein 9 antibody, anti oxysterol-binding protein-related protein 9 antibody

Reactivity

Human

Target

ORP9 / OSBPL9

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EPLLKRLGAAKH

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5 μg/ml, IHC-P: 2-4μg/ml

Molecular Weight

83.2; 62.5; 81.2; 70.2; 81.8; 84.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1) . All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

114883

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