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NET1/ARHGEF8 (C Terminus) Antibody

Goat polyclonal antibody to NET1

Product Specifications

Product Name Alternative

Anti NET1 antibody, anti ARHGEF8 antibody, anti NET1A antibody, anti neuroepithelial cell transforming gene 1 antibody, anti Rho guanine nucleotide exchange factor (GEF) 8 antibody, anti guanine nucleotide regulatory protein (oncogene) antibody, anti neuroepithelial cell transforming 1 antibody, anti RP11-116G8.1 antibody, anti Net1 proto-oncogene protein antibody, anti small GTP-binding protein regulator antibody

Reactivity

Human

Target

NET1 / ARHGEF8 (C Terminus)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DKALSGGKRKETLV

Field of Research

Epigenetics, Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, IHC-P: 1-2μg/ml

Molecular Weight

67.7; 61.9

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave bands at approx 85kDa in Human Placenta and a very strong band at approx 40-50kDa in Human Skeletal Muscle lysates after 0.1 μg/ml staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7kDa according to NP_001040625.1 and of 61.9kDa according to NP_005854.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Prediction Reactivity

Mouse

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

10276

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