Goat anti-POLL / POLkappa Antibody
Goat polyclonal antibody to POLL
Product Specifications
Product Name Alternative
Anti POLL antibody, anti BETA-N antibody, anti POL-KAPPA antibody, anti polymerase (DNA directed), lambda antibody, anti polymerase (DNA-directed), lambda antibody, anti DNA polymerase kappa (DNA polymerase beta-N) antibody, anti RP11-529I10.1 antibody, anti BETAN antibody, anti FLJ46002 antibody, anti POLKAPPA antibody, anti DNA polymerase beta-2 antibody, anti DNA polymerase kappa (DNA polymerase beta-N) antibody, anti DNA polymerase lamda2 antibody, anti DNA-directed DNA polymerase lambda antibody, anti OTTHUMP00000020323 antibody, anti pol beta2 antibody, anti pol lambda antibody
Reactivity
Human
Target
POLL / POLkappa
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
LGLPYREPAERDW
Field of Research
Epigenetics & Chromatin; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5-1 μg/ml
Molecular Weight
63.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
27343
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