Biocytin

Biocytin is a conjugate of D-biotin and L-lysine, where the carboxylate of D-biotin is coupled with the -amine of L-lysine via a secondary amide bond. Biocytin is a classical neuroanatomical tracer commonly used to map brain connectivity. Biocytin is used as a versatile marker in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays[1][2].

Product Specifications

CAS Number

[576-19-2]

Product Name Alternative

(+) -Biocytin

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Endogenous Metabolite

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Neurological Disease

Assay Protocol

https://www.medchemexpress.com/Biocytin.html

Purity

99.91

Solubility

H2O : 50 mg/mL (ultrasonic)

Smiles

N[C@@H](CCCCNC(CCCC[C@@H]1SC[C@]([C@]1([H])N2)([H])NC2=O)=O)C(O)=O

Molecular Formula

C16H28N4O4S

Molecular Weight

372.48

Precautions

H302, H315, H319, H335

References & Citations

[1]Roth G, et al. Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde, and intracellular biocytin labeling study. J Comp Neurol. 2004 Oct 4;478 (1) :35-61.|[2]Mishra A, et al. Improved neuronal tract tracing with stable biocytin-derived neuroimaging agents. ACS Chem Neurosci. 2010 Feb 17;1 (2) :129-38.|[3]MacNeil MA, et al. Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J Comp Neurol. 2008 Jan 1;506 (1) :6-15.