FOXB1/FKH5 Antibody
Goat polyclonal antibody to FOXB1
Product Specifications
Product Name Alternative
Anti FOXB1 antibody, anti forkhead box B1 antibody, anti FKH5 antibody, anti HFKH-5 antibody
Reactivity
Human, Mouse
Target
FOXB1 / FKH5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
TSPASALHSVAVH
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 0.1-0.3ug/ml
Molecular Weight
35
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave no signal but low background in Human Brain extracts at up to 1 μg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysatesExperiment Notes: Immunocytochemistry: Anonymous customer found nuclear staining at lumbar levels of the Mouse Spinal Cord at embryonic day 12.5. Recommended concentration 0.1-0.3ug/ml.
Prediction Reactivity
Bovine, Canine, Porcine, Rat
Tested Applications
ELISA, ICC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
27023
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