Nitric Oxide Colorimetric Detection Kit
This kit offers two assays. The first quantitatively measures endogenous Nitrite. In the second, Nitrate is converted to Nitrite using Nitrate Reductase and Total Nitric Oxide is measured. Nitrate levels can be calculated by performing both assays.
Product Specifications
Field of Research
Pharmacology & Drug Discovery
Assay Type
Detection
Sample Type
Water, buffers, serum, plasma, urine, saliva, tissue
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Reconstitute ThioBright Green with 500 μL DMSO to produce a 200X stock concentrate. 3. Add ThioBright Green to each sample at 1:200. For example, add 5 μL to 995 μL cultured cells. 4. Incubate 15-30 minutes. 5. Optional: Spin and pellet cells, remove supernatants, and gently resuspend in Assay Buffer or other isotonic buffer. 6. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody. 7. Analyze with a flow cytometer. ThioBright Green excites at 490 nm and emits at 525 nm.
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