Hydrogen Peroxide Fluorescent Detection Kit

1. Prepare Assay Buffer; dilute Assay Buffer Concentrate 1:5 in diH2O. 2. Prepare samples; dilute > 1:10 in Assay Buffer. 3. Prepare standard curve and zero (blank) standard. 4. Add 50 μL blanks, standards, and samples to plate. 5. Add 25 μL Colorimetric H2O2 Detection Substrate to plate. 6. Prepare HRP, dilute Horseradish Peroxidase Concentrate 1:50 in Assay Buffer. 7. Add 25 μL HRP Preparation to plate. 8. Incubate 15 minutes at RT. 9. Read absorbance of the plate at 560 nm.