Hydrogen Peroxide Fluorescent Detection Kit
Our Hydrogen Peroxide Fluorescent Detection Kit is designed to quantitatively measure H2O2 in a variety of samples. This kit is validated for use in fresh urine, buffers, and tissue culture media samples. It is species independent.
Product Specifications
Field of Research
Pharmacology & Drug Discovery
Assay Type
Detection
Sample Type
Fresh urine, buffers, and tissue culture media (TCM)
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare Assay Buffer; dilute Assay Buffer Concentrate 1:5 in diH2O. 2. Prepare samples; dilute > 1:10 in Assay Buffer. 3. Prepare standard curve and zero (blank) standard. 4. Add 50 μL blanks, standards, and samples to plate. 5. Add 25 μL Colorimetric H2O2 Detection Substrate to plate. 6. Prepare HRP, dilute Horseradish Peroxidase Concentrate 1:50 in Assay Buffer. 7. Add 25 μL HRP Preparation to plate. 8. Incubate 15 minutes at RT. 9. Read absorbance of the plate at 560 nm.
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