SR-FLIVO In vivo Poly Caspase Assay
A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a SR dye and a FMK reactive entity.
Product Specifications
Field of Research
Cell Biology
Sample Type
Whole live animal, excised tissue
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Dilute cellular wash buffer 1:10 with diH2O, 3. Reconstitute FLISP with 50 μL DMSO, 4. Dilute FLISP 1:5 by adding 200 μL PBS, 5. Add 10 μL FLISP to each sample (~500 μL aliquot of cultured cells), 6. Incubate ~ 1 hour. 7. Remove media and wash cells: add wash buffer and spin cells (twice) ; or add fresh media and incubate 1 hour, 8. If desired, label with additional stains, such as Hoechst, PI, or an antibody, 9. If desired, fix or embed cells, 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLISP excites at 488 nm and emits at 520 nm.
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