SAE2/UBA2 Antibody

Goat polyclonal to SAE2

Product Specifications

Product Name Alternative

Anti UBA2 antibody, anti SAE2 antibody, anti SUMO-1 activating enzyme subunit 2 antibody

Reactivity

Human, Mouse

Target

SAE2 / UBA2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ALSRGLPRELAEA

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, IHC-P: 10 μg/ml

Molecular Weight

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments showed an approx. 35kDa band in Human Testis and Brain extracts at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 74KDa according to NP_005490. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Prediction Reactivity

Bovine

Tested Applications

ELISA, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.