HighFidelity ATTO643 PCR Labeling Kit

HighFidelity ATTO643 PCR Labeling Kit is designed to produce ran-domly ATTO643-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-ATTO-643 is efficiently incorporated into DNA as substi-tute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpoly-merase and a proofreading enzyme. 50% dUTP-ATTO-643 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-ATTO-643/dTTP ratio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 μl each (50% dUTP-ATTO-643 substitution, 100 μM dATP/dGTP/dCTP, 50 μM dTTP, 50 μM dUTP-ATTO-643) .