Taq Core Kit

Taq Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexi-bility. Taq Polymerase is recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping. The buffer system guarantees robust and reliable amplification results in almost all PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium-and magnesium-ions to ensure high specificity and minimal by-product formation without the need of additional optimization steps. Ruby Buffer contains gel loading buffer and an inherent red dye allowing the direct loading of the PCR product into the gel. The red dye allows an easy visual control during PCR set-up and in combination with the density reagent the direct loading of the reaction product into the gel. The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease (proof-reading) activity.