What is the catalog number for HighFidelity GREEN PCR Labeling Testkit?

The catalog number for HighFidelity GREEN PCR Labeling Testkit is orb692240.

What sizes are available for HighFidelity GREEN PCR Labeling Testkit?

HighFidelity GREEN PCR Labeling Testkit is available in the following size(s): 1 Kit.

Is HighFidelity GREEN PCR Labeling Testkit in stock?

HighFidelity GREEN PCR Labeling Testkit is currently made to order / not held in immediate stock. Contact us for lead time.

HighFidelity GREEN PCR Labeling Testkit

HighFidelity GREEN PCR Labeling Testkit is designed to produce randomly Fluorescein-, ATTO488-and AF488-modified DNA probes by PCR to find the optimal label for the green emission wavelength range. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. All labeled-dUTPs are efficiently incorporated into DNA as sub-stitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taq polymerase and a proofreading enzyme. 50% labeled-dUTP substi-tution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of labeled-dUTP/dTTP ra-tio however, can easily be achieved with the single nucleotide format.

Product Specifications

Product Name Alternative

Preparation of FAMX-, ATTO488- and AZDye488-labeled DNA probes by PCR

Field of Research

Molecular Biology

Storage Conditions

Store at -20 °C

Notes

For research use only.

Applications Notes

Spectroscopic Propertie: Fluorescein: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), ATTO488: λexc 500 nm, λem 520 nm, ε 90.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), AF488: λexc 494 nm, λem 515 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .