E2-EPF/UBE2S Antibody

Goat polyclonal antibody to UBE2S

Product Specifications

Product Name Alternative

Anti E2-EPF antibody, anti ubiquitin carrier protein antibody, anti ubiquitin-protein ligase antibody, anti ubiquitin-conjugating enzyme E2-24 kD antibody, anti UBE2S antibody, anti ubiquitin-conjugating enzyme E2S antibody, anti E2EPF antibody, anti EPF5 antibody, anti ubiquitin carrier protein S antibody, anti ubiquitin-protein ligase S antibody

Reactivity

Human

Target

E2-EPF / UBE2S

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NSNVENLPPHIIR

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, IHC-P: 10ug/ml

Molecular Weight

23.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35-40kDa band observed in Human Liver lysate at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 25kDa according to NP_055316. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.