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Goat anti-TRIM35 / MAIR Antibody

Goat polyclonal antibody to TRIM35

Product Specifications

Product Name Alternative

Anti TRIM35 antibody, anti MAIR antibody, anti KIAA1098 antibody, anti MGC17233 antibody, anti tripartite motif-containing 35 antibody

Reactivity

Human

Target

TRIM35 / MAIR

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ERSPDVSPGPSRS

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml

Molecular Weight

56.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 25-30kDa band observed in human testis and human brain lysates at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted sizes of approx. 57kDa and 24kDa according to NP_055881and NP_741983. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

23087

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