Goat anti-TRIM35 / MAIR Antibody
Goat polyclonal antibody to TRIM35
Product Specifications
Product Name Alternative
Anti TRIM35 antibody, anti MAIR antibody, anti KIAA1098 antibody, anti MGC17233 antibody, anti tripartite motif-containing 35 antibody
Reactivity
Human
Target
TRIM35 / MAIR
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
ERSPDVSPGPSRS
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml
Molecular Weight
56.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 25-30kDa band observed in human testis and human brain lysates at 1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted sizes of approx. 57kDa and 24kDa according to NP_055881and NP_741983. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
23087
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