MURF2/TRIM55 Antibody

Goat polyclonal antibody to TRIM55

Product Specifications

Product Name Alternative

Anti TRIM55 antibody, anti RNF29 antibody, anti MURF-2 antibody, anti ring finger protein 29 antibody, anti for muscle specific ring finger 2 antibody, anti tripartite motif-containing 55 antibody, anti muscle specific ring finger 2 antibody

Reactivity

Human

Target

MURF2 / TRIM55

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HIFSFSWLNSLNE

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

60.5; 50.8; 26.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 85kDa band in human skeletal muscle lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.5kDa according to NP_908973.1. The 85kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.