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Goat anti-CARD6 Antibody

Goat polyclonal antibody to CARD6

Product Specifications

Product Name Alternative

Anti CARD6 antibody, anti caspase recruitment domain family, member 6 antibody, anti caspase recruitment domain protein 6 antibody, anti CINCIN1 antibody, anti CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation antibody

Reactivity

Human

Target

CARD6

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ATESTPSEIIERER

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, IHC-P: 5-10μg/ml

Molecular Weight

116

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10ug/ml.WB: Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

84674

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