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Goat anti-SSA1 / Ro52 Antibody

Goat polyclonal antibody to TRIM21

Product Specifications

Product Name Alternative

Anti TRIM21 antibody, anti tripartite motif-containing 21 antibody, anti RNF81, RO52, SSA, SSA1 antibody, anti 52kD Ro/SSA autoantigen antibody, anti 52kDa ribonucleoprotein autoantigen SS-A/Ro antibody, anti Sicca syndrome antigen A antibody, anti Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro) antibody, anti Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) antibody, anti tripartite motif protein TRIM21 antibody, anti tripartite motif protein 21 antibody

Reactivity

Human

Target

SSA1 / Ro52

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

CPLNIGSQGSTDY

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.2-1 μg/ml, IHC-P: 10ug/ml

Molecular Weight

54.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 38kDa and 25kDa in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 54.2kDa according to NP_003132.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, FC, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6737

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