Goat anti-SSA1 / Ro52 Antibody
Goat polyclonal antibody to TRIM21
Product Specifications
Product Name Alternative
Anti TRIM21 antibody, anti tripartite motif-containing 21 antibody, anti RNF81, RO52, SSA, SSA1 antibody, anti 52kD Ro/SSA autoantigen antibody, anti 52kDa ribonucleoprotein autoantigen SS-A/Ro antibody, anti Sicca syndrome antigen A antibody, anti Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro) antibody, anti Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro) antibody, anti tripartite motif protein TRIM21 antibody, anti tripartite motif protein 21 antibody
Reactivity
Human
Target
SSA1 / Ro52
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
CPLNIGSQGSTDY
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.2-1 μg/ml, IHC-P: 10ug/ml
Molecular Weight
54.2
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave bands at approx 38kDa and 25kDa in Human Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 54.2kDa according to NP_003132.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6737
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