What is the catalog number for EDD1/HYD Antibody?

The catalog number for EDD1/HYD Antibody is orb18490.

What sizes are available for EDD1/HYD Antibody?

EDD1/HYD Antibody is available in the following size(s): 100 µg.

Is EDD1/HYD Antibody in stock?

EDD1/HYD Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

EDD1/HYD Antibody

Goat polyclonal antibody to UBR5

Product Specifications

Product Name Alternative

Anti UBR5 antibody, anti EDD1 antibody, anti E3 ubiquitin protein ligase, HECT domain containing, 1 antibody, anti DD5 antibody, anti EDD antibody, anti HYD antibody, anti KIAA0896 antibody, anti progestin induced protein antibody, anti ubiquitin-protein ligase antibody, anti E3 identified by differential display antibody, anti hyperplastic discs protein homolog antibody, anti ubiquitin protein ligase E3 component n-recognin 5 antibody, anti FLJ11310 antibody, anti MGC57263 antibody

Reactivity

Human

Target

EDD1 / HYD

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

LAIKTKNFGFV

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, IHC-P: 1-2 μg/ml

Molecular Weight

309

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35-40kDa band observed in Human Testis, Human Skeletal Muscle, A549 and A431 lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 322kDa according to NP_056986 (furthermore, our SDS PAGE/western blotting protocol is not optimized for detection of proteins as large as the predicted) . Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Prediction Reactivity

Bovine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.