EDD1/HYD Antibody

Anti UBR5 antibody, anti EDD1 antibody, anti E3 ubiquitin protein ligase, HECT domain containing, 1 antibody, anti DD5 antibody, anti EDD antibody, anti HYD antibody, anti KIAA0896 antibody, anti progestin induced protein antibody, anti ubiquitin-protein ligase antibody, anti E3 identified by differential display antibody, anti hyperplastic discs protein homolog antibody, anti ubiquitin protein ligase E3 component n-recognin 5 antibody, anti FLJ11310 antibody, anti MGC57263 antibody

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35-40kDa band observed in Human Testis, Human Skeletal Muscle, A549 and A431 lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 322kDa according to NP_056986 (furthermore, our SDS PAGE/western blotting protocol is not optimized for detection of proteins as large as the predicted) . Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.