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Goat anti-MURF3 / TRIM54 Antibody

Goat polyclonal antibody to TRIM54

Product Specifications

Product Name Alternative

Anti TRIM54 antibody, anti MURF-3 antibody, anti tripartite motif-containing 54 antibody, anti RNF30 antibody, anti MURF antibody, anti ring finger protein 30 antibody, anti muscle-specific RING-finger protein 3 antibody, anti muscle-specific RING-finger protein homolog antibody, anti OTTHUMP00000122598 antibody

Reactivity

Human

Target

MURF3 / TRIM54

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GSAGPEEERPDGP

Field of Research

Stem Cell & Developmental Biology; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

44.8; 40.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Experiments gave bands at approx 45kDa and 38kDa in human skeletal muscle lysate after 1 μg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to NP_912730. Recommended concentration: 1-3 μg/ml.

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

57159

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