HRSV (B) Glycoprotein G Protein, His Tag

The two major glycoproteins on the surface of the RSV virion, the attachment glycoprotein (G) and the fusion (F) glycoprotein, control the initial phases of infection. The central region of the G protein contains a 13-amino acid highly conserved domain, partially overlapping the cysteine noose domain with 4 cysteines linked 1–4 and 2–3, followed by a highly basic heparin-binding domain (HBD) . The HBD is the likely attachment site for heparan sulfate (HS) found on the surface of most cells. A peptide from the G protein HBD (amino acids 184–198) binds efficiently to HEp-2 cells and inhibits RSV infection.

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 27.6 kDa. The protein migrates as 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized HRSV (B) Glycoprotein G Protein, His Tag (Cat. No. GLG-V5144) at 1 μg/mL (100 μL/well) can bind Anti-Respiratory Syncytial Virus G Glycoprotein Antibody, Mouse IgG1 with a linear range of 0.2-8 ng/mL (QC tested).