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Glutathione peroxidase 1 (GPx-1) Antibody

Our Anti-Glutathione peroxidase 1 (GPx-1) sheep polyclonal primary antibody detects human and rat Glutathione peroxidase 1 (GPx-1), and is whole serum. It is validated for use in ELISA, IHC-Frozen, IHC-Paraffin-embedded, WB.

Product Specifications

Product Name Alternative

GSHPx-1; GPx-1; Cellular glutathione peroxidase; GPX1

UniProt

P07203

Reactivity

Human, Rat

Immunogen

A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human lutathione peroxidase 1 (GPx-1) conjugated to diphtheria toxin has been used as the immunogen.

Target

Glutathione peroxidase 1 (GPx-1)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 1:10000-1:100000; IHC: 1:2000-1:20000; ELISA: 1:2000-1:20000

Form

Lyophilized

Molecular Weight

22 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Antigen retrieval has been used in testing but may not be necessary. This antiserum has extremely high titer, it stains human and rat brain microglia intensely and to some extent also stains neurons. Other tissues have not yet been tested. On Western blotting under reducing conditions recognizes a 22 kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes.

Tested Applications

ELISA, IHC, WB

Host or Source

Sheep

Isotype

Mixed

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