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Ancient ubiquitous protein 1 (AUP1) Antibody

Our Anti-Ancient ubiquitous protein 1 (AUP1) sheep polyclonal primary antibody detects bovine, human, rat, and sheep Ancient ubiquitous protein 1 (AUP1), and is whole serum. It is validated for use in IHC-Frozen, WB.

Product Specifications

Product Name Alternative

AUP1

UniProt

Q9Y679

Reactivity

Bovine, Human, Rat, Sheep

Immunogen

A synthetic peptide (HVFLVSCALPDSV) corresponding to the amino acids 48-60 of human ancient ubiquitous protein 1 conjugated to Blue carrier protein has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from bovine, mouse and rat.

Target

Ancient ubiquitous protein 1 (AUP1)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 1:500-1:6000; IHC: 1:500-1:6000

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC: Use at an amount of 10 μg/mL with an incubation time of 1-3 days at 4oC. This antiserum works in paraffin and 4% PFA fixed frozen sections. Penetration is the key to success. Over-fixed tissue is problematic. Not recommended for western blotting applications. Mouse monoclonal antibody M-1752-100 or rabbit polyclonal antibody R-1751-50 are excellent alternatives for Western blotting.

Tested Applications

IHC, WB

Host or Source

Sheep

Isotype

Mixed

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