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TrkB phospho Ser478/479 Antibody

Our Anti-Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479) rabbit polyclonal primary antibody detects chicken (predicted), human, mouse, and rat Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479), and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Product Specifications

Product Name Alternative

GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; pTrkB; phosphoTrkB; phospho-TrkB

UniProt

Q16620

Reactivity

Gallus, Human, Mouse, Rat

Immunogen

Synthetic peptide immunogen, SNDDDSA[pS]PLHHIS

Target

Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 0.5-2 μg/mL; IHC: 1-5 μg/mL

Form

Lyophilized

Molecular Weight

120 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. This antibody works on skim milk blocked membranes, however, best results are obtained with a equal mixture of 2.5% skim milk / 2.5% highly purified BSA as blocking reagent and antibody diluent. IHC: Antibody has been shown to work on PFA fixed, frozen sections. TBS is preferred for buffer preparation.

Prediction Reactivity

Gallus

Tested Applications

IHC, WB

Host or Source

Rabbit

Preservative

Lyophilized from 1X PBS, pH 7.2-7.6, with 0.1% trehalose

Isotype

IgG

Frequently Asked Questions

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