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TrkB phospho Tyr816/Y817 Antibody

Our Anti-TrkB, phospho Tyr816/Y817 (TrkB, pY816/817) rabbit polyclonal primary antibody detects chicken (predicted), human, mouse, and rat (predicted) TrkB, phospho Tyr816/Y817 (TrkB, pY816/817), and is affinity-purified. It is validated for use in WB.

Product Specifications

Product Name Alternative

GP145-TrkB; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Tropomyosin-related kinase B; pTrkB; phosphoTrkB; phospho-TrkB

UniProt

Q16620

Reactivity

Gallus, Human, Mouse, Rat

Immunogen

Synthetic peptide immunogen, AKASPV[pY]LDILG

Target

TrkB, phospho Tyr816/Y817 (TrkB, pY816/817)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 0.5-2 μg/mL

Form

Lyophilized

Molecular Weight

120 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

High skim milk concentration (5%) causes suppression of pY817 signal, suggesting that 5% skim milk should be avoided as blocking buffer and antibody diluent. Strongest signal is obtained in 5% BSA blocking buffer, however, many non-specific bands are present. An equal mixture of skim milk and BSA (2.5% each) appears to provide the best compromise between signal and noise. However, optimization of blocking condition is recommended for each particular sample, with excess of BSA over skim milk likely to be beneficial for best results.

Prediction Reactivity

Gallus, Rat

Tested Applications

WB

Host or Source

Rabbit

Preservative

PBS, pH 7.2-7.6, 0.1% trehalose, without preservatives.

Isotype

IgG

Frequently Asked Questions

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