Brain-derived neurotrophic factor (BDNF) Antibody

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human and other mammals (predicted) Brain-derived neurotrophic factor (BDNF), and is affinity-purified. It is validated for use in ELISA.

Product Specifications

Product Name Alternative

Brain-derived neurotrophic factor; Abrineurin

UniProt

P23560

Reactivity

Human

Immunogen

Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.

Target

Brain-derived neurotrophic factor (BDNF)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 0.2-1 μg/mL

Form

Lyophilized

Molecular Weight

14 kDa, 32 kDa (See application details.)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. R-1707-100 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (M-1744-50/100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG) . Flow Cytometry: ~2 μg per 10^6 cells, methanol fixation. Note: R-1707-100 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF.