Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) Antibody
Our Anti-Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A) rabbit polyclonal primary antibody detects human and rat Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A), and is whole serum. It is validated for use in ICC, WB.
Product Specifications
Product Name Alternative
Microtubule-associated proteins 1A/1B light chain 3A; MAP1A/MAP1B LC3 A; MAP1A/1B light chain 3 A; MAP1 light chain 3-like protein 1; Microtubule-associated protein 1 light chain 3 alpha; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; APG8a; MAP1LC3A
UniProt
Q9H492
Reactivity
Human, Rat
Immunogen
A synthetic peptide (RSFADRCKEVQQI) corresponding to the N-terminal of human MAP1LC3 A protein conjugated to Blue Carrier Protein has been used as the immunogen. The sequence is homologous with mouse and rat MAP1LC3 A.
Target
Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A)
Clonality
Polyclonal
Conjugation
Unconjugated
Dilution
WB: 1:500; IHC: 1:100-1:1000; ICC: 1:100-1:1000
Form
Lyophilized
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
IHC: 4% PFA fixed or 2% PLP fixation, ICC: acetone fixed specimens recommended. WB: R-146-100 requires samples to be denatured ONLY by boiling in SDS solution, not reduced. No signal is achieved using reduced samples. (Samples treated with DTT or Beta mercaptoethanol) . Overnight 4 degrees is recommended for primary antibody dilution in WB.
Tested Applications
ICC, WB
Host or Source
Rabbit
Isotype
Mixed
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