Tyrosine Hydroxylase (TH) Antibody

A synthetic peptide (PRFIGRRQSLIEDARK) as part of human Tyrosine Hydroxylase (63-78) conjugated to KLH has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from TH protein in rat (31-47) .

This antibody is for detection of tyrosine hydroxylase (TH) containing neurons exhibiting an intense labelling with a negligible background. This antiserum has proven useful for staining of catecholaminergic neurons. It stains dendritic processes and fine nerve terminals. We recommend mouse or rat brain containing catecholaminergic neurons as a positive control for this antibody, for example brain stem or striatum. Western blotting (WB) : Antibody has been tested on RIPA-extracted PC12 cell lysate and shown to be specific for TH (~60 kDa) . Tissue homogenates show a higher level of non-specific binding and presence of uncharacterized bands. Affinity-purified anti-TH antibody (R-148-50) is recommended for tissue homogenates.