Beta-nerve growth factor (Beta-NGF) Antibody

A concentration of 1-3 μg/mL is recommended for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 μg/mL (ED50) . This antibody was tested on cultured sensory neurons supported by 100 ng/mL of purified mouse beta NGF. Be advised that 2 μg/mL will neutralize 100 ng/mL of mouse NGF. The higher 10 μg/mL is only recommended if the concentration of NGF being used is higher than 100 ng/mL such as the 200 or 500 ng/mL that is occasionally used in some culture systems. This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.