Brain-derived neurotrophic factor (BDNF) Antibody
Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is whole serum. It is validated for use in ELISA, IHC-Frozen, WB.
Product Specifications
Product Name Alternative
Brain-derived neurotrophic factor; Abrineurin; proBDNF
UniProt
P23560
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
Target
Brain-derived neurotrophic factor (BDNF)
Clonality
Polyclonal
Conjugation
Unconjugated
Dilution
ELISA: 1:1000-1:5000 (detection only)
Form
Lyophilized
Molecular Weight
14, 18, 28, 32 kDa (see application details)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western Blotting: This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate) . Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014) .
Prediction Reactivity
Mammal
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Isotype
Mixed
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