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Choline O-acetyltransferase (ChAT) Antibody

Our Anti-Choline O-acetyltransferase (ChAT) rabbit polyclonal primary antibody detects guinea pig, mouse, pig, rabbit, and rat Choline O-acetyltransferase (ChAT), and is whole serum. It is validated for use in ELISA, ICC, IHC-Frozen, IHC-Paraffin-embedded.

Product Specifications

Product Name Alternative

Choline O-acetyltransferase; CHOACTase; Choline acetylase

UniProt

P13222

Reactivity

Guinea pig, Mouse, Porcine, Rabbit, Rat

Immunogen

A synthetic peptide (GLFSSYRLPGHTQDTLVAQKSS) as a part of porcine ChAT protein (aa: 167-188) conjugated to KLH

Target

Choline O-acetyltransferase (ChAT)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

IHC: 1:400-1:2000; ICC: 1:400-1:2000

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IHC/ICC on 4% PFA or formalin fixed frozen sections; paraffin sections can be more difficult and require more extensive antigen recovery methods. 1:400-1:2000 depending up detection and incubation times ELISA: direct antigen ELISA This antiserum will superbly stain both cell bodies and nerve terminal, and works particularly well in enteric and peripheral neurons.

Tested Applications

ELISA, ICC, IHC

Host or Source

Rabbit

Isotype

Mixed

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