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Neurotrophin Antibody Kit

Neurotrophin antibody kit of isotype controls containing a collection of our popular sheep antibodies raised against BDNF.

Product Specifications

UniProt

P20783, P34130, P23560, P01139

Reactivity

Human, Mouse, Rat

Immunogen

BDNF: recombinant human BDNF NGF: native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE) NT3: recombinant human NT3 NT4/5: recombinant human NT4

Target

Neurotrophins

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 1-10 μg/mL; IHC: 1-10 μg/mL; ELISA: 1-10 μg/mL

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunohistochemistry: 1-10 μg/mL. BDNF antibody works best on Zamboni's fixed, frozen tissue, and is not recommended for paraffin-embedded tissue. ELISA: 1-10 μg/mL. Western Blotting: 1-10 μg/mL. Western blotting is not recommended for the BDNF antibody. Inhibition of biological activity in vitro/in vivo: Expected ED50 (in vivo) values are in the range of 2-10 μg/mL. The NGF antibody completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. The negative control (normal sheep IgG) should be used at the same concentration as the neurotrophin antibodies.

Tested Applications

IHC, NeA

Host or Source

Sheep

Preservative

Lyophilized from PBS, pH 7.4, without preservatives.

Isotype

IgG

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