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NGFR/p75 neurotrophin receptor (p75NTR) Antibody

Selected Variant

GTR18793205

Size: 100 µg

Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects human, mouse, and rat NGFR/p75 neurotrophin receptor (p75NTR), and is IgG. It is validated for use in FC, ICC, IHC-Frozen, Immunopanning, IP, WB.

Product Specifications

Product Name Alternative

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271

UniProt

P08138

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.

Target

NGFR/p75 neurotrophin receptor (p75NTR)

Clonality

Monoclonal

Clone

8J2

Conjugation

Unconjugated

Dilution

WB: 0.5-2.0 μg/mL, non-reducing conditions only (no DTT or beta-mercaptoethanol) .; ICC: 1-5 μg/mL; IP: Lysate dependent. 10 ug per 200-500 ug total protein

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 5-20 μg/mL. Immunopanning: 1-5 μg/mL. ICC: Staining is strongest in non-fixed cells, light fixation is tolerable. IHC: fresh, acetone fixed sections only, epitope is fixation sensitive. Not suitable in formalin-fixed, paraffin (FFPE) embedded tissues.

Tested Applications

FC, ICC, IHC, IP, WB

Host or Source

Mouse

Preservative

Lyophilized from a solution containing PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives.

Isotype

IgG2a

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