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Spectrin alpha chain, non-erythrocytic 1 Antibody

Our Anti-Spectrin alpha chain, non-erythrocytic 1 mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Spectrin alpha chain, non-erythrocytic 1, and is affinity-purified. It is validated for use in FC, ICC, IHC-Frozen, WB.

Product Specifications

Product Name Alternative

SPTAN1; alpha II spectrin; alpha Fodrin; Spectrin, non-erythroid alpha chain; SPTA2

UniProt

Q13813

Reactivity

Bovine, Human, Mouse, Porcine, Rat

Immunogen

This antibody was raised against a recombinant construct containing the seventh, eight and ninth repeats (amino acids 676-1043) of human alpha-II Spectrin. The 9th spectrin repeat also includes a Src-homology 3 domain. This construct was expressed in and purified from E. coli.

Target

Spectrin alpha chain, non-erythrocytic 1

Clonality

Monoclonal

Clone

3D7

Conjugation

Unconjugated

Dilution

WB: 1:5000-1:10000; IHC: 1:1000-1:2000; ICC: 1:1000-1:2000

Form

Lyophilized

Molecular Weight

240 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

For Flow Cytometry, use ~ 2 μg antibody per ~10^6 cells. Optimal concentrations/dilutions should be determined by the end-user.

Tested Applications

FC, ICC, IHC, WB

Host or Source

Mouse

Preservative

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Isotype

IgG1

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